An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Inhibition of USP1, a new partner of Bcr-Abl, results in decrease of Bcr-Abl level in K562 cells.

Antonenko SV, Telegeev GD

To analyze interaction of ubiquitin specific peptidase 1 (USP1) with Bcr-Abl and to assess the relation between USP1 functional activity and Bcr-Abl expression in K562 chronic myeloid leukemia cells.The interaction between USP1 and Bcr-Abl in K562 cells was analyzed by co-immunoprecipitation, Western blot analysis, and confocal microscopy.A direct interaction between Bcr-Abl oncoprotein and USP1 protein in K562 cells was established by co-immunoprecipitation. Immunofluorescence analysis ... [more]

Exp Oncol Dec. 01, 2019; 42(2);109-114 [Pubmed: 32602291]

Throughput

Low Throughput

Additional Notes

interaction between Bcr-Abl and USP1 detected using a Bcr antibody

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
USP1 BCR	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Antonenko SV (2020)	Low	-	BioGRID	2971733

Curated By

BioGRID

Interaction Summary

BCR

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activator activity [TAS]kinase activity [TAS]protein binding [IPI]protein tyrosine kinase activity [TAS]

Gene Ontology Cellular Component

USP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase activity [TAS]protein binding [IPI]ubiquitin thiolesterase activity [IDA]ubiquitin-specific protease activity [IDA, IMP]