BAIT

AURKB

AIK2, AIM-1, AIM1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5, aurkb-sv1, aurkb-sv2
aurora kinase B
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Deubiquitylase UCHL3 regulates bi-orientation and segregation of chromosomes during mitosis.

Jerabkova K, Liao Y, Kleiss C, Fournane S, Durik M, Agote-Aran A, Brino L, Sedlacek R, Sumara I

Equal segregation of chromosomes during mitosis ensures euploidy of daughter cells. Defects in this process may result in an imbalance in the chromosomal composition and cellular transformation. Proteolytic and non-proteolytic ubiquitylation pathways ensure directionality and fidelity of mitotic progression but specific mitotic functions of deubiquitylating enzymes (DUBs) remain less studied. Here we describe the role of the DUB ubiquitin carboxyl-terminal ... [more]

FASEB J Dec. 01, 2019; 34(9);12751-12767 [Pubmed: 32738097]

Throughput

  • Low Throughput

Curated By

  • BioGRID