BAIT

NSP2

ORF1ab, ORF1ab-nsp2, SARS-CoV2 nsp2, SARS-CoV-2 nsp2, R1AB_SARS2, PRO_0000449620, GU280_gp01_nsp2
Non-structural protein 2
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

POR

CPR, CYPOR, P450R
P450 (cytochrome) oxidoreductase
Arachidonic Acid Pathway Project
GO Process (3)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.

Gupta M, Azumaya CM, Moritz M, Pourmal S, Diallo A, Merz GE, Jang GM, Bouhaddou M, Fossati A, Brilot AF, Diwanji D, Hernandez E, Herrera N, Kratochvil HT, Lam VL, Li F, Li Y, Nguyen HC, Nowotny C, Owens TW, Peters JK, Rizo AN, Schulze-Gahmen U, Smith AM, Young ID, Yu Z, Asarnow D, Billesbolle C, Campbell MG, Chen J, Chen K-H, Chio US, Dickinson MS, Doan L, Jin M, Kim K, Li J, Li Y-L, Linossi E, Liu Y, Lo M, Lopez J, Lopez KE, Mancino A, Moss FR, Paul MD, Pawar KI

The SARS-CoV-2 protein Nsp2 has been implicated in a wide range of viral processes, but its exact functions, and the structural basis of those functions, remain unknown. Here, we report an atomic model for full-length Nsp2 obtained by combining cryo-electron microscopy with deep learning-based structure prediction from AlphaFold2. The resulting structure reveals a highly-conserved zinc ion-binding site, suggesting a role ... [more]

Status: Preliminary Report

Throughput

  • High Throughput

Additional Notes

  • Bait = Mutant SARS-CoV-2 Nsp2 (SARS2_nsp2_D23Y_R27C)
  • Bait = Mutant SARS-CoV-2 Nsp2 (SARS2_nsp2_G262V_G265V)
  • Bait = Mutant SARS-CoV-2 Nsp2 (SARS2_nsp2_T85I)
  • Bait = Wild type SARS-CoV-2 Nsp2

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NSP2 POR
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.711BioGRID
2652474
NSP2 POR
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.711BioGRID
2875486
NSP2 POR
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High1BioGRID
3209188

Curated By

  • BioGRID