An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.

Gupta M, Azumaya CM, Moritz M, Pourmal S, Diallo A, Merz GE, Jang GM, Bouhaddou M, Fossati A, Brilot AF, Diwanji D, Hernandez E, Herrera N, Kratochvil HT, Lam VL, Li F, Li Y, Nguyen HC, Nowotny C, Owens TW, Peters JK, Rizo AN, Schulze-Gahmen U, Smith AM, Young ID, Yu Z, Asarnow D, Billesbolle C, Campbell MG, Chen J, Chen K-H, Chio US, Dickinson MS, Doan L, Jin M, Kim K, Li J, Li Y-L, Linossi E, Liu Y, Lo M, Lopez J, Lopez KE, Mancino A, Moss FR, Paul MD, Pawar KI

The SARS-CoV-2 protein Nsp2 has been implicated in a wide range of viral processes, but its exact functions, and the structural basis of those functions, remain unknown. Here, we report an atomic model for full-length Nsp2 obtained by combining cryo-electron microscopy with deep learning-based structure prediction from AlphaFold2. The resulting structure reveals a highly-conserved zinc ion-binding site, suggesting a role ... [more]

Status: Preliminary Report

Throughput

High Throughput

Additional Notes

Bait = Mutant SARS-CoV-2 Nsp2 (SARS2_nsp2_D23Y_R27C)
Bait = Mutant SARS-CoV-2 Nsp2 (SARS2_nsp2_G262V_G265V)
Bait = Mutant SARS-CoV-2 Nsp2 (SARS2_nsp2_K330D_K337D)
Bait = Mutant SARS-CoV-2 Nsp2 (SARS2_nsp2_T85I)
Bait = Wild type SARS-CoV-2 Nsp2

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NSP2 FKBP15	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gordon DE (2020)	High	0.9881	BioGRID	2652477
NSP2 FKBP15	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gordon DE (2020)	High	0.9881	BioGRID	2875489
NSP2 FKBP15	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Zheng YX (2021)	High	-	BioGRID	3458157
NSP2 FKBP15	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Laurent E (2020)	High	-	BioGRID	2799564
NSP2 FKBP15	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Samavarchi-Tehrani P (2020)	High	29.41	BioGRID	2792032
NSP2 FKBP15	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	St-Germain JR (2020)	High	98	BioGRID	2806674
NSP2 FKBP15	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Zhang Y (2022)	High	-	BioGRID	3494917

Curated By

BioGRID

Interaction Summary

NSP2

FKBP15

Gene Ontology Biological Process

Gene Ontology Cellular Component