An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Nsp2 has the potential to be a drug target revealed by global identification of SARS-CoV-2 Nsp2-interacting proteins.

Zheng YX, Wang L, Kong WS, Chen H, Wang XN, Meng Q, Zhang HN, Guo SJ, Jiang HW, Tao SC

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)?has become a global health threat since December 2019, and there is still no highly effective drug to control the pandemic. To facilitate drug target identification for drug development, studies on molecular mechanisms, such as SARS-CoV-2 protein interactions, are urgently needed. In this study, we focused on Nsp2, a non-structural protein with largely unknown ... [more]

Acta Biochim Biophys Sin (Shanghai) Aug. 31, 2021; 53(9);1134-1141 [Pubmed: 34159380]

Throughput

High Throughput

Additional Notes

Affinity capture-MS using SARS-CoV-2 as the bait identified 84 interacting host proteins.
High confidence interactors had a SILAC heavy/light abundance ratio > 4 as the cutoff.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NSP2 FKBP15	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gordon DE (2020)	High	0.9881	BioGRID	2652477
NSP2 FKBP15	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gordon DE (2020)	High	0.9881	BioGRID	2875489
NSP2 FKBP15	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gupta M (2021)	High	-	BioGRID	2973108
NSP2 FKBP15	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Laurent E (2020)	High	-	BioGRID	2799564
NSP2 FKBP15	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Samavarchi-Tehrani P (2020)	High	29.41	BioGRID	2792032
NSP2 FKBP15	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	St-Germain JR (2020)	High	98	BioGRID	2806674
NSP2 FKBP15	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Zhang Y (2022)	High	-	BioGRID	3494917

Curated By

BioGRID

Interaction Summary

NSP2

FKBP15

Gene Ontology Biological Process

Gene Ontology Cellular Component