BAIT

INSL3

RLF, RLNL, ley-I-L
insulin-like 3 (Leydig cell)
GO Process (2)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

RXFP2

GPR106, GREAT, INSL3R, LGR8, LGR8.1, RXFPR2
relaxin/insulin-like family peptide receptor 2
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

INSL3/Leydig insulin-like peptide activates the LGR8 receptor important in testis descent.

Kumagai J, Hsu SY, Matsumi H, Roh JS, Fu P, Wade JD, Bathgate RA, Hsueh AJ

Several orphan G protein-coupled receptors homologous to gonadotropin and thyrotropin receptors have recently been identified and named as LGR4-8. INSL3, also known as Leydig insulin-like peptide or relaxin-like factor, is a relaxin family member expressed in testis Leydig cells and ovarian theca and luteal cells. Male mice mutant for INSL3 exhibit cryptorchidism or defects in testis descent due to abnormal ... [more]

J. Biol. Chem. Aug. 30, 2002; 277(35);31283-6 [Pubmed: 12114498]

Throughput

  • Low Throughput

Curated By

  • BioGRID