BAIT

DUN1

serine/threonine protein kinase DUN1, L000000531, YDL101C
Cell-cycle checkpoint serine-threonine kinase; required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; Mec1p and Dun1p function in same pathway to regulate both dNTP pools and telomere length; also regulates postreplicative DNA repair
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

RSP5

MDP1, MUT2, NPI1, SMM1, UBY1, NEDD4 family E3 ubiquitin-protein ligase, L000001054, L000001779, L000001220, YER125W
E3 ubiquitin ligase of NEDD4 family; regulates many cellular processes including MVB sorting, heat shock response, transcription, endocytosis, ribosome stability; mutant tolerates aneuploidy; autoubiquitinates; ubiquitinates Sec23p and Sna3p; deubiquitinated by Ubp2p; activity regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; human homolog implicated in Liddle syndrome
GO Process (33)
GO Function (3)
GO Component (9)

Gene Ontology Biological Process

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Dun1, a Chk2-related kinase, is the central regulator of securin-separase dynamics during DNA damage signaling.

Yam CQX, Chia DB, Shi I, Lim HH, Surana U

The DNA damage checkpoint halts cell cycle progression in G2 in response to genotoxic insults. Central to the execution of cell cycle arrest is the checkpoint-induced stabilization of securin-separase complex (yeast Pds1-Esp1). The checkpoint kinases Chk1 and Chk2 (yeast Chk1 and Rad53) are thought to critically contribute to the stability of securin-separase complex by phosphorylation of securin, rendering it resistant ... [more]

Nucleic Acids Res Dec. 19, 2019; 48(11);6092-6107 [Pubmed: 32402080]

Throughput

  • Low Throughput

Ontology Terms

  • protein/peptide modification (APO:0000131)
  • cell cycle progression (APO:0000253)

Additional Notes

  • Figure 7
  • rsp5-1

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RSP5 DUN1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
DUN1 RSP5
Dosage Lethality
Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

High-BioGRID
798014

Curated By

  • BioGRID