BAIT
ANAPC2
APC2, RP11-350O14.5
anaphase promoting complex subunit 2
GO Process (9)
GO Function (0)
GO Component (3)
Gene Ontology Biological Process
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- mitotic cell cycle [TAS]
- mitotic spindle assembly checkpoint [TAS]
- negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- positive regulation of synapse maturation [ISS]
- positive regulation of synaptic plasticity [ISS]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- protein K11-linked ubiquitination [IDA]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
HSD17B4
DBP, MFE-2, MPF-2, PRLTS1, SDR8C1
hydroxysteroid (17-beta) dehydrogenase 4
GO Process (15)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- alpha-linolenic acid metabolic process [TAS]
- androgen metabolic process [IDA]
- bile acid biosynthetic process [TAS]
- bile acid metabolic process [TAS]
- cellular lipid metabolic process [TAS]
- estrogen metabolic process [IDA]
- fatty acid beta-oxidation [IDA]
- fatty acid beta-oxidation using acyl-CoA oxidase [TAS]
- medium-chain fatty-acyl-CoA metabolic process [IDA]
- metabolic process [IDA]
- osteoblast differentiation [IDA]
- oxidation-reduction process [IDA, IMP]
- small molecule metabolic process [TAS]
- unsaturated fatty acid metabolic process [TAS]
- very long-chain fatty-acyl-CoA metabolic process [IDA]
Gene Ontology Molecular Function- 17-beta-hydroxysteroid dehydrogenase (NAD+) activity [IDA]
- 3-hydroxyacyl-CoA dehydrogenase activity [IDA, IMP, TAS]
- 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity [TAS]
- long-chain-enoyl-CoA hydratase activity [IDA, TAS]
- protein homodimerization activity [IDA]
- receptor binding [IPI]
- 17-beta-hydroxysteroid dehydrogenase (NAD+) activity [IDA]
- 3-hydroxyacyl-CoA dehydrogenase activity [IDA, IMP, TAS]
- 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity [TAS]
- long-chain-enoyl-CoA hydratase activity [IDA, TAS]
- protein homodimerization activity [IDA]
- receptor binding [IPI]
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 55.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID