BAIT

SHS1

SEP7, septin SHS1, YDL225W
Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

CLN2

cyclin CLN2, L000000358, YPL256C
G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN2 has a paralog, CLN1, that arose from the whole genome duplication
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The septins function in G1 pathways that influence the pattern of cell growth in budding yeast.

Egelhofer TA, Villen J, McCusker D, Gygi SP, Kellogg DR

The septins are a conserved family of proteins that have been proposed to carry out diverse functions. In budding yeast, the septins become localized to the site of bud emergence in G1 but have not been thought to carry out important functions at this stage of the cell cycle. We show here that the septins function in redundant mechanisms that ... [more]

PLoS ONE Apr. 24, 2008; 3(4);e2022 [Pubmed: 18431499]

Throughput

  • Low Throughput

Ontology Terms

  • bud morphology (APO:0000212)
  • bud neck morphology (APO:0000056)
  • inviable (APO:0000112)

Additional Notes

  • The experiment involved a cln1 cln2 shs1 triple mutant.

Curated By

  • BioGRID