BAIT
DCTN1
DAP-150, DP-150, P135
dynactin 1
GO Process (9)
GO Function (1)
GO Component (8)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- activation of signaling protein activity involved in unfolded protein response [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- cellular protein metabolic process [TAS]
- endoplasmic reticulum unfolded protein response [TAS]
- mitotic cell cycle [TAS]
- mitotic nuclear division [NAS]
- nervous system development [NAS]
- retrograde transport, endosome to Golgi [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CENPE
CENP-E, KIF10, MCPH13, PPP1R61
centromere protein E, 312kDa
GO Process (12)
GO Function (4)
GO Component (14)
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- attachment of mitotic spindle microtubules to kinetochore [IMP]
- blood coagulation [TAS]
- kinetochore assembly [NAS]
- metabolic process [IMP]
- microtubule-based movement [IBA, TAS]
- mitotic cell cycle [IBA, TAS]
- mitotic chromosome movement towards spindle pole [TAS]
- mitotic metaphase plate congression [IMP]
- positive regulation of protein kinase activity [IMP]
- regulation of mitotic metaphase/anaphase transition [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- chromosome [IDA]
- chromosome, centromeric region [IDA]
- condensed chromosome outer kinetochore [TAS]
- condensed chromosome, centromeric region [IDA]
- cytoplasm [IDA]
- cytosol [TAS]
- kinesin complex [IBA]
- kinetochore [IDA]
- membrane [IDA]
- microtubule [IDA]
- microtubule cytoskeleton [IDA]
- midbody [IDA]
- mitotic spindle midzone [IDA]
- nucleus [IMP]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 620.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID