BAIT
GOLGA2
GM130, RP11-395P17.5
golgin A2
GO Process (2)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
STK24
HEL-S-95, MST3, MST3B, STE20, STK3, RP11-111L24.5
serine/threonine kinase 24
GO Process (11)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- apoptotic process [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- execution phase of apoptosis [IMP]
- intrinsic apoptotic signaling pathway in response to oxidative stress [IMP]
- negative regulation of cell migration [IMP]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA]
- regulation of axon regeneration [IMP]
- response to hydrogen peroxide [IDA]
- signal transduction [TAS]
- signal transduction by phosphorylation [IBA]
Gene Ontology Molecular Function
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 11.95 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID