BAIT

CKS1

CATALYTIC SUBUNIT OF CYCLIN-DEPENDENT KINASE, CKS1AT, P10CKS1AT, T1E2.12, T1E2_12, cyclin-dependent kinase-subunit 1, AT2G27960
cyclin-dependent kinases regulatory subunit 1
GO Process (3)
GO Function (2)
GO Component (2)

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

CDKB1;1

CDC2-LIKE GENE, CDC2B, CYCLIN-DEPENDENT KINASE B1;1, P34(CDC2)-LIKE PROTEIN, AT3G54180
cyclin-dependent kinase B1-1
GO Process (4)
GO Function (3)
GO Component (2)
Arabidopsis thaliana (Columbia)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A tandem affinity purification-based technology platform to study the cell cycle interactome in Arabidopsis thaliana.

Van Leene J, Stals H, Eeckhout D, Persiau G, Van De Slijke E, Van Isterdael G, De Clercq A, Bonnet E, Laukens K, Remmerie N, Henderickx K, De Vijlder T, Abdelkrim A, Pharazyn A, Van Onckelen H, Inze D, Witters E, De Jaeger G

Defining protein complexes is critical to virtually all aspects of cell biology because many cellular processes are regulated by stable protein complexes, and their identification often provides insights into their function. We describe the development and application of a high throughput tandem affinity purification/mass spectrometry platform for cell suspension cultures to analyze cell cycle-related protein complexes in Arabidopsis thaliana. Elucidation ... [more]

Mol. Cell Proteomics Jul. 01, 2007; 6(7);1226-38 [Pubmed: 17426018]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CKS1 CDKB1;1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
CDKB1;1 CKS1
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

High-BioGRID
-
CKS1 CDKB1;1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
CDKB1;1 CKS1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID