BAIT

CKS1

CATALYTIC SUBUNIT OF CYCLIN-DEPENDENT KINASE, CKS1AT, P10CKS1AT, T1E2.12, T1E2_12, cyclin-dependent kinase-subunit 1, AT2G27960
cyclin-dependent kinases regulatory subunit 1
GO Process (3)
GO Function (2)
GO Component (2)

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

CDKB1;1

CDC2-LIKE GENE, CDC2B, CYCLIN-DEPENDENT KINASE B1;1, P34(CDC2)-LIKE PROTEIN, AT3G54180
cyclin-dependent kinase B1-1
GO Process (4)
GO Function (3)
GO Component (2)
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

The Arabidopsis Cks1At protein binds the cyclin-dependent kinases Cdc2aAt and Cdc2bAt.

De Veylder L, Segers G, Glab N, Casteels P, Van Montagu M, Inze D

In Arabidopsis, two cyclin-dependent kinases (CDK), Cdc2aAt and Cdc2bAt, have been described. Here, we have used the yeast two-hybrid system to identify Arabidopsis proteins interacting with Cdc2aAt. Three different clones were isolated, one of which encodes a Suc1/Cks1 homologue. The functionality of the Arabidopsis Suc1/Cks1 homologue, designed Cks1At, was demonstrated by its ability to rescue the temperature-sensitive cdc2-L7 strain of ... [more]

FEBS Lett. Aug. 04, 1997; 412(3);446-52 [Pubmed: 9276444]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CKS1 CDKB1;1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
CKS1 CDKB1;1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
CDKB1;1 CKS1
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

High-BioGRID
-
CDKB1;1 CKS1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID