BAIT
KRT8
CARD2, CK-8, CK8, CYK8, K2C8, K8, KO
keratin 8
GO Process (0)
GO Function (2)
GO Component (5)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CLASP1
MAST1
cytoplasmic linker associated protein 1
GO Process (14)
GO Function (4)
GO Component (12)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- axon guidance [TAS]
- cell division [IDA]
- establishment of spindle orientation [IDA, IGI, IMP]
- establishment or maintenance of cell polarity [NAS]
- exit from mitosis [IMP]
- microtubule anchoring [IMP]
- microtubule bundle formation [IMP]
- microtubule cytoskeleton organization [IGI]
- microtubule nucleation [IMP]
- microtubule organizing center organization [IMP]
- mitotic cell cycle [TAS]
- negative regulation of microtubule depolymerization [IGI, IMP]
- negative regulation of microtubule polymerization or depolymerization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 10.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID