BAIT
LAMTOR1
C11orf59, PDRO, Ragulator1, p18, p27RF-Rho, PP7157
late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
GO Process (15)
GO Function (3)
GO Component (8)
Gene Ontology Biological Process
- cell growth [IMP]
- cellular protein localization [IMP]
- cellular response to amino acid stimulus [IMP]
- cholesterol homeostasis [IMP]
- endosome localization [ISS]
- endosome organization [ISS]
- lysosome localization [ISS]
- lysosome organization [ISS]
- positive regulation of GTPase activity [IDA]
- positive regulation of MAPK cascade [ISS]
- positive regulation of TOR signaling [IMP]
- regulation of cholesterol efflux [IMP]
- regulation of cholesterol esterification [IMP]
- regulation of cholesterol import [IMP]
- regulation of receptor recycling [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PSEN1
AD3, FAD, PS-1, PS1, S182
presenilin 1
GO Process (15)
GO Function (6)
GO Component (27)
Gene Ontology Biological Process
- Notch receptor processing [IBA, TAS]
- amyloid precursor protein catabolic process [IBA, TAS]
- beta-amyloid metabolic process [IBA]
- calcium ion transmembrane transport [IMP]
- canonical Wnt signaling pathway [IBA]
- endoplasmic reticulum calcium ion homeostasis [IDA, IGI]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- membrane protein ectodomain proteolysis [IDA]
- negative regulation of apoptotic process [IDA]
- positive regulation of catalytic activity [IDA]
- protein processing [IDA]
- regulation of phosphorylation [IDA]
- single organismal cell-cell adhesion [IMP]
- smooth endoplasmic reticulum calcium ion homeostasis [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- Z disc [IBA]
- apical plasma membrane [IBA]
- axon [IBA]
- cell cortex [IBA]
- cell surface [IBA]
- centrosome [IDA]
- ciliary rootlet [IBA]
- dendritic shaft [IBA]
- endoplasmic reticulum [IDA]
- gamma-secretase complex [IDA]
- growth cone [IBA]
- integral component of membrane [TAS]
- integral component of plasma membrane [IDA]
- kinetochore [IDA]
- lysosomal membrane [IBA]
- membrane [IDA]
- membrane raft [IBA, IDA]
- mitochondrial inner membrane [IBA]
- mitochondrion [IDA]
- neuromuscular junction [IBA]
- neuronal cell body [IBA]
- nuclear membrane [IDA]
- nuclear outer membrane [IDA]
- perinuclear region of cytoplasm [IBA]
- rough endoplasmic reticulum [IDA]
- smooth endoplasmic reticulum [IDA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 25.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID