BAIT
PAFAH1B1
LIS1, LIS2, MDCR, MDS, PAFAH
platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
GO Process (24)
GO Function (8)
GO Component (11)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- acrosome assembly [ISS]
- actin cytoskeleton organization [ISS]
- adult locomotory behavior [IMP]
- brain morphogenesis [IMP]
- cerebral cortex development [IMP]
- corpus callosum morphogenesis [IMP]
- establishment of mitotic spindle orientation [IMP]
- hippocampus development [ISS]
- layer formation in cerebral cortex [ISS]
- learning or memory [ISS]
- microtubule cytoskeleton organization [ISS]
- microtubule organizing center organization [IMP]
- microtubule-based process [IDA]
- mitotic cell cycle [TAS]
- neuroblast proliferation [ISS]
- neuromuscular process controlling balance [IMP]
- neuron migration [IMP, ISS]
- platelet activating factor metabolic process [ISS]
- regulation of Rho GTPase activity [ISS]
- retrograde axon cargo transport [ISS]
- synaptic transmission [ISS]
- transmission of nerve impulse [ISS]
- vesicle transport along microtubule [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PSMC4
MIP224, RPT3, S6, TBP-7, TBP7
proteasome (prosome, macropain) 26S subunit, ATPase, 4
GO Process (23)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- ATP catabolic process [TAS]
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- RNA metabolic process [TAS]
- anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- apoptotic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- mitotic cell cycle [TAS]
- negative regulation of apoptotic process [TAS]
- negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- protein polyubiquitination [TAS]
- proteolysis [TAS]
- regulation of apoptotic process [TAS]
- regulation of cellular amino acid metabolic process [TAS]
- regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]
- small molecule metabolic process [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
A proximity-dependent biotinylation map of a human cell.
Compartmentalization is a defining characteristic of eukaryotic cells, and partitions distinct biochemical processes into discrete subcellular locations. Microscopy1 and biochemical fractionation coupled with mass spectrometry2-4 have defined the proteomes of a variety of different organelles, but many intracellular compartments have remained refractory to such approaches. Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of ... [more]
Nature Jun. 02, 2021; (); [Pubmed: 34079125]
Quantitative Score
- 11.67 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- SAINTexpress (v.3.6.1) was used to identify proximity interactions and those with a Bayesian FDR =< 0.01 were considered high confidence. The score represents the fold change of the average spectral count in sample replicates relative to the average in control replicates.
Curated By
- BioGRID