BAIT
MCM5
BOB1, CDC46, MCM DNA helicase complex subunit MCM5, L000000279, YLR274W
Component of the Mcm2-7 hexameric helicase complex; MCM complex is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
GO Process (8)
GO Function (5)
GO Component (7)
Gene Ontology Biological Process
- DNA replication initiation [IMP]
- chromatin silencing at telomere [IMP]
- double-strand break repair via break-induced replication [IMP]
- establishment of chromatin silencing [IMP]
- negative regulation of chromatin silencing at telomere [IMP]
- nuclear DNA replication [IMP]
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication [IDA, IPI]
- regulation of DNA-dependent DNA replication initiation [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
CLB6
B-type cyclin CLB6, L000000354, YGR109C
B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1; CLB6 has a paralog, CLB5, that arose from the whole genome duplication
GO Process (3)
GO Function (1)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Interplay between S-cyclin-dependent kinase and Dbf4-dependent kinase in controlling DNA replication through phosphorylation of yeast Mcm4 N-terminal domain.
Cyclin-dependent (CDK) and Dbf4-dependent (DDK) kinases trigger DNA replication in all eukaryotes, but how these kinases cooperate to regulate DNA synthesis is largely unknown. Here, we show that budding yeast Mcm4 is phosphorylated in vivo during S phase in a manner dependent on the presence of five CDK phosphoacceptor residues within the N-terminal domain of Mcm4. Mutation to alanine of ... [more]
Mol. Biol. Cell May. 01, 2008; 19(5);2267-77 [Pubmed: 18321994]
Throughput
- Low Throughput
Ontology Terms
- phenotype: mitotic cell cycle (APO:0000072)
- phenotype: resistance to chemicals (APO:0000087)
- phenotype: cold sensitivity (APO:0000148)
- phenotype: inviable (APO:0000112)
Additional Notes
- bob1 clb5 clb6 triple mutant is sensitive to cold and hydroxyurea
- genetic complex
Curated By
- BioGRID