BAIT
ANXA1
ANX1, LPC1, RP11-71A24.1
annexin A1
GO Process (12)
GO Function (8)
GO Component (11)
Gene Ontology Biological Process
- alpha-beta T cell differentiation [ISS]
- cellular component movement [TAS]
- cellular response to glucocorticoid stimulus [IDA]
- inflammatory response [TAS]
- keratinocyte differentiation [IDA]
- negative regulation of apoptotic process [TAS]
- negative regulation of interleukin-8 secretion [IMP]
- neutrophil clearance [IMP]
- neutrophil homeostasis [IMP]
- peptide cross-linking [IDA]
- positive regulation of vesicle fusion [IDA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
KRT8
CARD2, CK-8, CK8, CYK8, K2C8, K8, KO
keratin 8
GO Process (0)
GO Function (2)
GO Component (5)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Lipocortin 1 co-associates with cytokeratins 8 and 18 in A549 cells via the N-terminal domain.
An affinity chromatography strategy was used to search for proteins in A549 cells which interact with the N-terminus of lipocortin 1 (annexin 1). Using the biologically active fragment Lc13-25 as the affinity ligand, two proteins of molecular weight (m.w.) 52 and 48kDa were extracted. Affinity blots of these proteins bound iodinated Lc13-25. Partial tryptic digests of these proteins were analysed ... [more]
Biochim. Biophys. Acta Jan. 02, 1998; 1401(1);39-51 [Pubmed: 9459484]
Throughput
- Low Throughput
Curated By
- BioGRID