Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Protein interactome reveals converging molecular pathways among autism disorders.

Sakai Y, Shaw CA, Dawson BC, Dugas DV, Al-Mohtaseb Z, Hill DE, Zoghbi HY

To uncover shared pathogenic mechanisms among the highly heterogeneous autism spectrum disorders (ASDs), we developed a protein interaction network that identified hundreds of new interactions among proteins encoded by ASD-associated genes. We discovered unexpectedly high connectivity between SHANK and TSC1, previously implicated in syndromic autism, suggesting that common molecular pathways underlie autistic phenotypes in distinct syndromes. ASD patients were more ... [more]

Sci Transl Med Jun. 08, 2011; 3(86);86ra49 [Pubmed: 21653829]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

CDKL5

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]Rac GTPase binding [ISS]kinase activity [IDA]protein kinase activity [IDA]protein serine/threonine kinase activity [TAS]

Gene Ontology Cellular Component

TACC3