An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

BPA modulates the WDR5/TET2 complex to regulate ER? expression in eutopic endometrium and drives the development of endometriosis.

Xue W, Yao X, Ting G, Ling J, Huimin L, Yuan Q, Chun Z, Ming Z, Yuanzhen Z

Overexpression of estrogen receptor ? (ER?) in endometrium contributes to endometriosis (EM) pathogenesis. Trimethylation of the H3 lysine (K) 4 (H3K4me3) in promoters is strongly correlated with gene expression. This study aimed to explore the effects of bisphenol A (BPA) exposure on EM development from the perspective of the regulation of ER? expression in eutopic endometrium via the H3K4me3-related epigenetic ... [more]

Environ Pollut Jan. 01, 2021; 268(Pt B);115748 [Pubmed: 33022573]

Quantitative Score

391.0 [Mascot Score]

Throughput

High Throughput

Additional Notes

Affinity capture-MS was carried out to identify high-confidence protein interactions. Mascot Scores are reported here.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
WDR5 TCP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hauri S (2016)	High	-	BioGRID	-
TCP1 WDR5	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Havugimana PC (2022)	High	-	BioGRID	3439207
WDR5 TCP1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Raisch J (2023)	High	-	BioGRID	3571515

Curated By

BioGRID

Interaction Summary

WDR5

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone acetyltransferase activity (H4-K16 specific) [IDA]histone acetyltransferase activity (H4-K5 specific) [IDA]histone acetyltransferase activity (H4-K8 specific) [IDA]histone methyltransferase activity (H3-K4 specific) [IDA]protein binding [IPI]

Gene Ontology Cellular Component

TCP1