A2M
Gene Ontology Biological Process
- blood coagulation [TAS]
- blood coagulation, intrinsic pathway [TAS]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- negative regulation of complement activation, lectin pathway [IDA]
- negative regulation of endopeptidase activity [IDA]
- platelet activation [TAS]
- platelet degranulation [TAS]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
IL10
Gene Ontology Biological Process
- B cell differentiation [NAS]
- B cell proliferation [NAS]
- cell-cell signaling [IC]
- cellular response to lipopolysaccharide [NAS]
- cytoplasmic sequestering of NF-kappaB [NAS]
- hemopoiesis [TAS]
- inflammatory response [IDA]
- leukocyte chemotaxis [TAS]
- negative regulation of B cell proliferation [IDA]
- negative regulation of MHC class II biosynthetic process [TAS]
- negative regulation of T cell proliferation [NAS]
- negative regulation of apoptotic process [NAS]
- negative regulation of cytokine secretion involved in immune response [IDA]
- negative regulation of interferon-alpha biosynthetic process [NAS]
- negative regulation of interleukin-6 production [IDA]
- negative regulation of membrane protein ectodomain proteolysis [IDA]
- positive regulation of B cell apoptotic process [IDA]
- positive regulation of JAK-STAT cascade [IBA]
- positive regulation of cytokine secretion [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- receptor biosynthetic process [IDA]
- regulation of gene expression [IDA]
- regulation of isotype switching [NAS]
- response to glucocorticoid [IDA]
- response to molecule of bacterial origin [IDA]
- type 2 immune response [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Interleukin-4 and IL-10 bind covalently to activated human alpha2-macroglobulin by a mechanism that requires Cys949.
Alpha2-macroglobulin (alpha2M) functions as an extracellular carrier of diverse cytokines, including transforming growth factor-beta1 (TGF-beta1), that expresses anti-inflammatory activities. The results presented here demonstrate that interleukin-10 (IL-10) and IL-4, which also regulate the inflammatory response, bind to alpha2M. Unlike TGF-beta, IL-4 and IL-10 bind almost exclusively to the receptor-recognized, or activated, form of alpha2M. Purified IL-4-alpha2M complexes were predominantly covalent ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| IL10 A2M | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | High | - | BioGRID | 1504781 |
Curated By
- BioGRID