Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Functional analysis and the molecular dissection of zinc-fingers and homeoboxes 1 (ZHX1).

Yamada K, Kawata H, Matsuura K, Shou Z, Hirano S, Mizutani T, Yazawa T, Yoshino M, Sekiguchi T, Kajitani T, Miyamoto K

Zinc-fingers and homeoboxes 1 (ZHX1) is a protein that interacts with the activation domain of the A subunit of nuclear factor-Y. The function of ZHX1, as a transcription factor, was characterized and their domains were mapped. To determine the nuclear localization signal, expression vectors, in which various truncated forms of ZHX1 were fused to the C-terminal of green fluorescence protein ... [more]

Biochem. Biophys. Res. Commun. Sep. 20, 2002; 297(2);368-74 [Pubmed: 12237128]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ZHX1 ZHX1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hirano S (2002)	Low	-	BioGRID	-
ZHX1 ZHX1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Yamada K (2002)	Low	-	BioGRID	-
ZHX1 ZHX1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yamada K (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ZHX1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein heterodimerization activity [IDA]sequence-specific DNA binding transcription factor activity [IDA]transcription corepressor activity [IDA]

Gene Ontology Cellular Component