Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Detection of protein-protein interactions among lens crystallins in a mammalian two-hybrid system assay.

Fu L, Liang JJ

alpha-Crystallin consists of two subunits, alphaA and alphaB, and each can form an oligomer by itself or with the other. The aggregation arises from interdomain interactions. However, it is not known whether such interactions also exist among alpha-, beta-, and gamma-crystallins. This heterogeneous crystallin interaction is far weaker than the homogeneous crystallin interaction and is difficult to detect by conventional ... [more]

J. Biol. Chem. Feb. 08, 2002; 277(6);4255-60 [Pubmed: 11700327]

Throughput

Low Throughput

Additional Notes

mammalian two-hybrid

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CRYBB2 CRYAA	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Fu L (2002)	Low	-	BioGRID	-
CRYBB2 CRYAA	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fu L (2002)	Low	-	BioGRID	303557
CRYAA CRYBB2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fu L (2003)	Low	-	BioGRID	722956

Curated By

BioGRID

Interaction Summary

CRYAA

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein binding [IPI]unfolded protein binding [IMP, IPI]

Gene Ontology Cellular Component

CRYBB2

Gene Ontology Molecular Function

identical protein binding [IPI]structural molecule activity [NAS]

Two-hybrid