Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Alteration of protein-protein interactions of congenital cataract crystallin mutants.

Fu L, Liang JJ

A recent study demonstrated the presence of protein-protein interactions among lens crystallins in a mammalian cell two-hybrid system assay and speculated about the significance of these interactions for protein solubility and lens transparency. The current study extends those findings to the following crystallin genes involved in some congenital cataracts: CRYAA (R116C), CRYAB (R120G), and CRYGC (T5P).A mammalian two-hybrid system was ... [more]

Invest. Ophthalmol. Vis. Sci. Mar. 01, 2003; 44(3);1155-9 [Pubmed: 12601044]

Throughput

Low Throughput

Additional Notes

mammalian two-hybrid system

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CRYBB2 CRYAA	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Fu L (2002)	Low	-	BioGRID	-
CRYAA CRYBB2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fu L (2002)	Low	-	BioGRID	303556
CRYBB2 CRYAA	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fu L (2002)	Low	-	BioGRID	303557

Curated By

BioGRID

Interaction Summary

CRYAA

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein binding [IPI]unfolded protein binding [IMP, IPI]

Gene Ontology Cellular Component

CRYBB2

Gene Ontology Molecular Function

identical protein binding [IPI]structural molecule activity [NAS]

Two-hybrid