NDE1
Gene Ontology Biological Process
- centrosome duplication [IMP]
- centrosome localization [IBA]
- cerebral cortex development [IGI, ISO]
- chromosome segregation [IBA]
- establishment of chromosome localization [IBA, ISO]
- establishment of mitotic spindle orientation [IBA, ISO]
- forebrain development [IMP]
- microtubule nucleation [IDA]
- microtubule organizing center organization [IMP]
- mitotic centrosome separation [IBA]
- neuroblast proliferation [IMP]
- neuron migration [IMP]
- vesicle transport along microtubule [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PAFAH1B1
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- acrosome assembly [ISS]
- actin cytoskeleton organization [ISS]
- adult locomotory behavior [IMP]
- brain morphogenesis [IMP]
- cerebral cortex development [IMP]
- corpus callosum morphogenesis [IMP]
- establishment of mitotic spindle orientation [IMP]
- hippocampus development [ISS]
- layer formation in cerebral cortex [ISS]
- learning or memory [ISS]
- microtubule cytoskeleton organization [ISS]
- microtubule organizing center organization [IMP]
- microtubule-based process [IDA]
- mitotic cell cycle [TAS]
- neuroblast proliferation [ISS]
- neuromuscular process controlling balance [IMP]
- neuron migration [IMP, ISS]
- platelet activating factor metabolic process [ISS]
- regulation of Rho GTPase activity [ISS]
- retrograde axon cargo transport [ISS]
- synaptic transmission [ISS]
- transmission of nerve impulse [ISS]
- vesicle transport along microtubule [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
LIS1 regulates CNS lamination by interacting with mNudE, a central component of the centrosome.
LIS1, a microtubule-associated protein, is required for neuronal migration, but the precise mechanism of LIS1 function is unknown. We identified a LIS1 interacting protein encoded by a mouse homolog of NUDE, a nuclear distribution gene in A. nidulans and a multicopy suppressor of the LIS1 homolog, NUDF. mNudE is located in the centrosome or microtubule organizing center (MTOC), and interacts ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NDE1 PAFAH1B1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| NDE1 PAFAH1B1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID