BAIT

RAD52

RP11-359B12.2

RAD52 homolog (S. cerevisiae)

GO Process (5)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

DNA recombinase assembly [TAS]

DNA recombination [IMP]

DNA repair [TAS]

double-strand break repair [TAS]

double-strand break repair via homologous recombination [TAS]

Gene Ontology Molecular Function

identical protein binding [IPI]
protein binding [IPI]

Gene Ontology Cellular Component

nucleoplasm [TAS]

CRISPR Database OMIM VEGA HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

UBE2I

C358B7.1, P18, UBC9, LA16c-358B7.1

ubiquitin-conjugating enzyme E2I

GO Process (8)

GO Function (9)

GO Component (6)

Gene Ontology Biological Process

cellular protein metabolic process [TAS]

cellular protein modification process [TAS]

negative regulation of transcription from RNA polymerase II promoter [IMP]

negative regulation of transcription, DNA-templated [IDA]

post-translational protein modification [TAS]

protein sumoylation [IDA, TAS]

protein ubiquitination [IBA]

ubiquitin-dependent protein catabolic process [TAS]

Gene Ontology Molecular Function

RING-like zinc finger domain binding [IPI]
SUMO transferase activity [IDA]
enzyme binding [IPI]
poly(A) RNA binding [IDA]
protein binding [IPI]
transcription factor binding [IPI]
ubiquitin protein ligase activity [IBA]
ubiquitin protein ligase binding [IBA]
ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleoplasm [TAS]

synaptonemal complex [TAS]

transferase complex [IDA]

CRISPR Database VEGA OMIM HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system.

Shen Z, Pardington-Purtymun PE, Comeaux JC, Moyzis RK, Chen DJ

The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the "bait" in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like ... [more]

Genomics Oct. 15, 1996; 37(2);183-6 [Pubmed: 8921390]

Throughput

Low Throughput

Curated By

BioGRID