BAIT
CYP2J2
CPJ2
cytochrome P450, family 2, subfamily J, polypeptide 2
GO Process (9)
GO Function (8)
GO Component (4)
Gene Ontology Biological Process
- arachidonic acid metabolic process [TAS]
- epoxygenase P450 pathway [IDA, TAS]
- exogenous drug catabolic process [IBA]
- icosanoid metabolic process [TAS]
- linoleic acid metabolic process [IDA]
- oxidation-reduction process [IBA]
- regulation of heart contraction [TAS]
- small molecule metabolic process [TAS]
- xenobiotic metabolic process [TAS]
Gene Ontology Molecular Function- arachidonic acid 11,12-epoxygenase activity [IDA]
- arachidonic acid 14,15-epoxygenase activity [IDA]
- arachidonic acid epoxygenase activity [IDA]
- heme binding [IBA]
- linoleic acid epoxygenase activity [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen [IBA]
- oxygen binding [IBA]
- steroid hydroxylase activity [IBA]
- arachidonic acid 11,12-epoxygenase activity [IDA]
- arachidonic acid 14,15-epoxygenase activity [IDA]
- arachidonic acid epoxygenase activity [IDA]
- heme binding [IBA]
- linoleic acid epoxygenase activity [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen [IBA]
- oxygen binding [IBA]
- steroid hydroxylase activity [IBA]
Gene Ontology Cellular Component
Homo sapiens
PREY
VPS28
vacuolar protein sorting 28 homolog (S. cerevisiae)
GO Process (10)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- endosomal transport [TAS]
- intracellular transport of virus [TAS]
- membrane organization [TAS]
- negative regulation of protein ubiquitination [IDA]
- protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IBA]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IC, IMP]
- viral life cycle [TAS]
- viral process [TAS]
- viral protein processing [TAS]
- virion assembly [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.998554836 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.998554836, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- BioPlex HCT HCT116 cells CompPASS score = 0.999945131, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID