VAMP2
Gene Ontology Biological Process
- Golgi to plasma membrane protein transport [ISS]
- calcium ion-dependent exocytosis [ISS]
- cellular protein metabolic process [TAS]
- cellular response to insulin stimulus [ISS]
- energy reserve metabolic process [TAS]
- eosinophil degranulation [IMP]
- exocytosis [IBA, TAS]
- glutamate secretion [TAS]
- long-term synaptic potentiation [ISS]
- membrane fusion [ISS]
- membrane organization [TAS]
- mucus secretion [IMP]
- natural killer cell degranulation [IMP]
- neurotransmitter secretion [TAS]
- neutrophil degranulation [IMP]
- positive regulation of intracellular protein transport [ISS]
- post-Golgi vesicle-mediated transport [TAS]
- protein complex assembly [ISS]
- protein transport [ISS]
- regulation of exocytosis [ISS]
- regulation of histamine secretion by mast cell [IMP]
- regulation of insulin secretion [TAS]
- regulation of vesicle-mediated transport [ISS]
- response to glucose [ISS]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
- synaptic vesicle exocytosis [ISS, TAS]
- vesicle fusion [IBA]
- vesicle-mediated transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IDA, TAS]
- clathrin-coated vesicle [IDA]
- clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane [TAS]
- clathrin-sculpted glutamate transport vesicle membrane [TAS]
- clathrin-sculpted monoamine transport vesicle membrane [TAS]
- cytoplasmic vesicle [ISS]
- endocytic vesicle membrane [TAS]
- extracellular vesicular exosome [IDA]
- integral component of plasma membrane [TAS]
- intracellular membrane-bounded organelle [ISS]
- membrane [IDA]
- neuron projection [ISS]
- neuron projection terminus [ISS]
- perinuclear region of cytoplasm [ISS]
- plasma membrane [ISS, TAS]
- secretory granule [ISS]
- secretory granule membrane [IDA, TAS]
- synapse [ISS]
- synaptic vesicle [IBA, ISS, TAS]
- synaptic vesicle membrane [ISS]
- synaptobrevin 2-SNAP-25-syntaxin-1a complex [ISS]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex [ISS]
- synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex [ISS]
- trans-Golgi network [ISS]
- zymogen granule membrane [ISS]
STX1A
Gene Ontology Biological Process
- cellular protein metabolic process [TAS]
- energy reserve metabolic process [TAS]
- glutamate secretion [TAS]
- intracellular protein transport [IBA]
- neurotransmitter secretion [TAS]
- regulation of insulin secretion [TAS]
- secretion by cell [IDA]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
- synaptic vesicle fusion to presynaptic membrane [IBA]
- vesicle docking [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
A stable interaction between syntaxin 1a and synaptobrevin 2 mediated by their transmembrane domains.
The proteins synaptobrevin (VAMP), SNAP-25 and syntaxin 1 are essential for neuronal exocytosis. They assemble into a stable ternary complex which is thought to initiate membrane fusion. In vitro, the transmembrane domains of syntaxin and synaptobrevin are not required for association. Here we report a novel interaction between synaptobrevin and syntaxin that requires the presence of the transmembrane domains. When ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
STX1A VAMP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
STX1A VAMP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
STX1A VAMP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
STX1A VAMP2 | Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex. | Low | - | BioGRID | - | |
VAMP2 STX1A | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | - | |
STX1A VAMP2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
VAMP2 STX1A | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
STX1A VAMP2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2711002 | |
STX1A VAMP2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID