BAIT
SETMAR
HsMar1, METNASE, Mar1
SET domain and mariner transposase fusion gene
GO Process (15)
GO Function (10)
GO Component (3)
Gene Ontology Biological Process
- DNA catabolic process, endonucleolytic [IDA, IMP]
- DNA double-strand break processing [IDA]
- DNA integration [IMP]
- cell proliferation [IMP]
- double-strand break repair via nonhomologous end joining [IDA, IMP]
- histone H3-K36 dimethylation [IMP]
- histone H3-K36 methylation [IDA, IMP]
- histone H3-K4 methylation [IDA]
- mitotic DNA integrity checkpoint [IMP]
- negative regulation of cell cycle arrest [IMP]
- negative regulation of chromosome organization [IDA]
- nucleic acid phosphodiester bond hydrolysis [IMP]
- positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity [IDA, IMP]
- positive regulation of double-strand break repair via nonhomologous end joining [IDA]
- replication fork processing [IMP]
Gene Ontology Molecular Function- DNA topoisomerase binding [IPI]
- double-stranded DNA binding [IMP]
- endonuclease activity [IDA, IMP]
- histone methyltransferase activity (H3-K36 specific) [IDA]
- histone methyltransferase activity (H3-K4 specific) [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- single-stranded DNA binding [IMP]
- single-stranded DNA endodeoxyribonuclease activity [IMP]
- structure-specific DNA binding [IDA, TAS]
- DNA topoisomerase binding [IPI]
- double-stranded DNA binding [IMP]
- endonuclease activity [IDA, IMP]
- histone methyltransferase activity (H3-K36 specific) [IDA]
- histone methyltransferase activity (H3-K4 specific) [IDA]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- single-stranded DNA binding [IMP]
- single-stranded DNA endodeoxyribonuclease activity [IMP]
- structure-specific DNA binding [IDA, TAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
PCBP2
HNRNPE2, HNRPE2, hnRNP-E2
poly(rC) binding protein 2
GO Process (8)
GO Function (5)
GO Component (7)
Gene Ontology Biological Process
- RNA splicing [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- mRNA metabolic process [NAS]
- mRNA splicing, via spliceosome [TAS]
- negative regulation of defense response to virus [IMP]
- negative regulation of type I interferon production [TAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.767365244 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.767365244, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID