BAIT
EDN3
ET-3, ET3, HSCR4, PPET3, WS4B, RP4-614C15.1
endothelin 3
GO Process (17)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
- artery smooth muscle contraction [IDA]
- blood circulation [TAS]
- cell surface receptor signaling pathway [IDA]
- cell-cell signaling [TAS]
- inositol phosphate-mediated signaling [IDA]
- multicellular organismal development [TAS]
- neutrophil chemotaxis [IDA]
- peptide hormone secretion [IDA]
- positive regulation of MAP kinase activity [IDA]
- positive regulation of heart rate [IDA]
- positive regulation of hormone secretion [IDA]
- positive regulation of leukocyte chemotaxis [IDA]
- positive regulation of mitosis [IDA]
- regulation of systemic arterial blood pressure by endothelin [IDA]
- signal transduction [TAS]
- vasoconstriction [IDA]
- vein smooth muscle contraction [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TUBB3
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4
tubulin, beta 3 class III
GO Process (4)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.843538273 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.843538273, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID