BAIT

DNAJC14

DNAJ, DRIP78, HDJ3, LIP6, hCG_2016179
DnaJ (Hsp40) homolog, subfamily C, member 14
GO Process (0)
GO Function (0)
GO Component (4)
Homo sapiens
PREY

DRD1

DADR, DRD1A
dopamine receptor D1
GO Process (22)
GO Function (4)
GO Component (2)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Regulation of transport of the dopamine D1 receptor by a new membrane-associated ER protein.

Bermak JC, Li M, Bullock C, Zhou QY

Many structural determinants for G protein-coupled receptor (GPCR) functions have been defined, but little is known concerning the regulation of their transport from the endoplasmic reticulum (ER) to the cell surface. Here we show that a carboxy-terminal hydrophobic motif, FxxxFxxxF, which is highly conserved among GPCRs, functions independently as an ER-export signal for the dopamine D1 receptor. A newly identified ... [more]

Nat. Cell Biol. May. 01, 2001; 3(5);492-8 [Pubmed: 11331877]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DNAJC14 DRD1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID