BAIT
PRKAA1
AMPK, AMPKa1
protein kinase, AMP-activated, alpha 1 catalytic subunit
GO Process (22)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
- activation of MAPK activity [NAS]
- cell cycle arrest [TAS]
- cellular response to glucose starvation [ISS]
- cellular response to nutrient levels [ISS]
- fatty acid homeostasis [ISS]
- glucose homeostasis [ISS]
- insulin receptor signaling pathway [TAS]
- lipid biosynthetic process [ISS]
- negative regulation of TOR signaling [ISS]
- negative regulation of apoptotic process [ISS]
- negative regulation of glucosylceramide biosynthetic process [NAS]
- negative regulation of lipid catabolic process [ISS]
- positive regulation of autophagy [ISS]
- positive regulation of cholesterol biosynthetic process [NAS]
- positive regulation of gene expression [IDA]
- positive regulation of glycolytic process [ISS]
- protein phosphorylation [IDA]
- regulation of circadian rhythm [ISS]
- regulation of energy homeostasis [ISS]
- response to gamma radiation [ISS]
- response to hypoxia [NAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DPYSL3
CRMP-4, CRMP4, DRP-3, DRP3, LCRMP, ULIP, ULIP-1
dihydropyrimidinase-like 3
GO Process (10)
GO Function (2)
GO Component (7)
Gene Ontology Biological Process
- actin crosslink formation [ISS]
- actin filament bundle assembly [ISS]
- axon guidance [TAS]
- cellular response to cytokine stimulus [ISS]
- negative regulation of cell migration [ISS]
- negative regulation of neuron projection development [ISS]
- positive regulation of filopodium assembly [ISS]
- positive regulation of neuron projection development [ISS]
- protein homooligomerization [ISS]
- response to axon injury [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.99965519 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.99965519, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID