BAIT
INS
IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10
insulin
GO Process (60)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- MAPK cascade [IDA]
- activation of protein kinase B activity [IDA]
- acute-phase response [IDA]
- alpha-beta T cell activation [IDA]
- cell-cell signaling [IC]
- cellular protein metabolic process [TAS]
- endocrine pancreas development [TAS]
- energy reserve metabolic process [TAS]
- fatty acid homeostasis [IMP]
- glucose homeostasis [IMP]
- glucose transport [IDA]
- insulin receptor signaling pathway [TAS]
- negative regulation of NAD(P)H oxidase activity [IDA]
- negative regulation of acute inflammatory response [IDA]
- negative regulation of fatty acid metabolic process [IMP]
- negative regulation of feeding behavior [IDA]
- negative regulation of gluconeogenesis [NAS]
- negative regulation of glycogen catabolic process [IMP]
- negative regulation of lipid catabolic process [IMP, NAS]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [NAS]
- negative regulation of protein catabolic process [IDA]
- negative regulation of protein oligomerization [IDA]
- negative regulation of protein secretion [IDA]
- negative regulation of proteolysis [IMP]
- negative regulation of respiratory burst involved in inflammatory response [IDA]
- negative regulation of vasodilation [NAS]
- positive regulation of DNA replication [IDA]
- positive regulation of MAPK cascade [IDA]
- positive regulation of NF-kappaB transcription factor activity [IDA]
- positive regulation of brown fat cell differentiation [TAS]
- positive regulation of cell differentiation [NAS]
- positive regulation of cell growth [NAS]
- positive regulation of cell migration [ISS]
- positive regulation of cell proliferation [IDA]
- positive regulation of cellular protein metabolic process [IMP]
- positive regulation of cytokine secretion [IDA]
- positive regulation of glucose import [IDA]
- positive regulation of glycogen biosynthetic process [IDA]
- positive regulation of glycolytic process [IDA, IMP]
- positive regulation of insulin receptor signaling pathway [IDA]
- positive regulation of lipid biosynthetic process [NAS]
- positive regulation of mitosis [IDA]
- positive regulation of nitric oxide biosynthetic process [NAS]
- positive regulation of nitric-oxide synthase activity [NAS]
- positive regulation of peptide hormone secretion [TAS]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of phosphatidylinositol 3-kinase signaling [IDA]
- positive regulation of protein autophosphorylation [ISS]
- positive regulation of protein kinase B signaling [IDA]
- positive regulation of respiratory burst [IDA]
- positive regulation of vasodilation [NAS]
- regulation of cellular amino acid metabolic process [IMP]
- regulation of insulin secretion [TAS]
- regulation of protein localization [IDA]
- regulation of protein secretion [IDA]
- regulation of transcription, DNA-templated [NAS]
- regulation of transmembrane transporter activity [IDA]
- small molecule metabolic process [TAS]
- wound healing [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
LAMA5
RP11-157P1.6
laminin, alpha 5
GO Process (13)
GO Function (2)
GO Component (9)
Gene Ontology Biological Process
- angiogenesis [NAS]
- cell differentiation [NAS]
- cell migration [IDA]
- cell proliferation [NAS]
- cell recognition [NAS]
- cytoskeleton organization [NAS]
- embryo development [NAS]
- endothelial cell differentiation [NAS]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- focal adhesion assembly [NAS]
- integrin-mediated signaling pathway [IMP]
- substrate adhesion-dependent cell spreading [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.807280479 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.807280479, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID