INSL5
Gene Ontology Cellular Component
ADAM17
Gene Ontology Biological Process
- B cell differentiation [ISS]
- JAK-STAT cascade involved in growth hormone signaling pathway [TAS]
- Notch receptor processing [IDA]
- PMA-inducible membrane protein ectodomain proteolysis [IDA, IMP]
- T cell differentiation in thymus [ISS]
- apoptotic process [TAS]
- apoptotic signaling pathway [TAS]
- cell adhesion [IDA]
- cell adhesion mediated by integrin [IDA]
- cell motility [ISS]
- collagen catabolic process [TAS]
- epidermal growth factor receptor signaling pathway [IDA, TAS]
- epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway [IMP]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- germinal center formation [ISS]
- membrane protein ectodomain proteolysis [IDA, IMP]
- membrane protein intracellular domain proteolysis [TAS]
- negative regulation of interleukin-8 production [IMP]
- negative regulation of transforming growth factor beta receptor signaling pathway [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- neutrophil mediated immunity [IC]
- positive regulation of T cell chemotaxis [IMP]
- positive regulation of cell growth [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of cellular component movement [ISS]
- positive regulation of chemokine production [IMP]
- positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle [IDA]
- positive regulation of epidermal growth factor-activated receptor activity [IDA, IMP]
- positive regulation of leukocyte chemotaxis [IC]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of transforming growth factor beta receptor signaling pathway [ISS]
- proteolysis [IDA]
- regulation of mast cell apoptotic process [ISS]
- response to drug [ISS]
- response to high density lipoprotein particle [IDA]
- response to hypoxia [IDA]
- response to lipopolysaccharide [IDA]
- spleen development [ISS]
- wound healing, spreading of epidermal cells [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Quantitative Score
- 0.915442749 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.915442749, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| INSL5 ADAM17 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9036 | BioGRID | 2258401 | |
| INSL5 ADAM17 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.5075 | BioGRID | 3261068 |
Curated By
- BioGRID