BAIT
GOT1
ASTQTL1, GIG18, cAspAT, cCAT
glutamic-oxaloacetic transaminase 1, soluble
GO Process (16)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- 2-oxoglutarate metabolic process [ISS]
- L-methionine biosynthetic process from methylthioadenosine [TAS]
- aspartate catabolic process [IDA]
- aspartate metabolic process [ISS]
- carbohydrate metabolic process [TAS]
- cellular amino acid biosynthetic process [TAS]
- cellular nitrogen compound metabolic process [TAS]
- cellular response to insulin stimulus [IEP]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- glutamate metabolic process [ISS]
- glycerol biosynthetic process [ISS]
- polyamine metabolic process [TAS]
- response to glucocorticoid [IEP]
- small molecule metabolic process [TAS]
- sulfur amino acid metabolic process [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
AKR1C2
AKR1C-pseudo, BABP, DD, DD-2, DD/BABP, DD2, DDH2, HAKRD, HBAB, MCDR2, SRXY8, TDD
aldo-keto reductase family 1, member C2
GO Process (13)
GO Function (7)
GO Component (0)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA]
- cellular response to jasmonic acid stimulus [IDA]
- cellular response to prostaglandin D stimulus [IDA]
- daunorubicin metabolic process [IMP]
- digestion [IDA]
- doxorubicin metabolic process [IMP]
- epithelial cell differentiation [IEP]
- oxidation-reduction process [IDA]
- positive regulation of cell proliferation [IDA]
- positive regulation of protein kinase B signaling [IDA]
- progesterone metabolic process [IDA]
- prostaglandin metabolic process [IDA]
- steroid metabolic process [IDA]
Gene Ontology Molecular Function- alditol:NADP+ 1-oxidoreductase activity [IDA]
- bile acid binding [IDA]
- carboxylic acid binding [IDA]
- ketosteroid monooxygenase activity [IDA]
- oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor [IDA]
- phenanthrene 9,10-monooxygenase activity [IDA]
- trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity [IDA]
- alditol:NADP+ 1-oxidoreductase activity [IDA]
- bile acid binding [IDA]
- carboxylic acid binding [IDA]
- ketosteroid monooxygenase activity [IDA]
- oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor [IDA]
- phenanthrene 9,10-monooxygenase activity [IDA]
- trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.973736729 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.973736729, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID