BAIT
GDF9
growth differentiation factor 9
GO Process (10)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
- BMP signaling pathway [IBA]
- SMAD protein signal transduction [IBA]
- cell development [IBA]
- female gamete generation [TAS]
- positive regulation of cell proliferation [IDA]
- positive regulation of pathway-restricted SMAD protein phosphorylation [IBA]
- regulation of MAPK cascade [IBA]
- regulation of apoptotic process [IBA]
- regulation of progesterone secretion [IDA, IMP]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
APBB1
FE65, MGC:9072, RIR
amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
GO Process (10)
GO Function (6)
GO Component (6)
Gene Ontology Biological Process
- axonogenesis [NAS]
- cell cycle arrest [ISS]
- cellular response to DNA damage stimulus [IDA]
- histone H4 acetylation [ISS]
- negative regulation of cell growth [ISS]
- negative regulation of thymidylate synthase biosynthetic process [ISS]
- positive regulation of apoptotic process [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of transcription, DNA-templated [ISS]
- signal transduction [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.825348762 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.825348762, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID