BAIT
SYP
MRX96, MRXSYP
synaptophysin
GO Process (7)
GO Function (2)
GO Component (3)
Gene Ontology Biological Process
- endocytosis [ISS]
- regulation of long-term neuronal synaptic plasticity [ISS]
- regulation of neuronal synaptic plasticity [IBA]
- regulation of opioid receptor signaling pathway [ISS]
- regulation of short-term neuronal synaptic plasticity [ISS]
- synaptic vesicle maturation [NAS]
- synaptic vesicle membrane organization [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CLN3
BTS, JNCL
ceroid-lipofuscinosis, neuronal 3
GO Process (30)
GO Function (2)
GO Component (20)
Gene Ontology Biological Process
- action potential [ISS]
- amyloid precursor protein catabolic process [IDA]
- arginine transport [IDA]
- associative learning [ISS]
- autophagic vacuole fusion [ISS]
- cellular amino acid metabolic process [ISS]
- ceramide metabolic process [IDA]
- cytosolic calcium ion homeostasis [ISS]
- galactosylceramide metabolic process [IMP]
- globoside metabolic process [IMP]
- glucosylceramide metabolic process [IMP]
- ionotropic glutamate receptor signaling pathway [ISS]
- lysosomal lumen acidification [IMP]
- lysosomal lumen pH elevation [IDA]
- lysosome organization [ISS]
- macroautophagy [NAS]
- membrane organization [ISS]
- negative regulation of apoptotic process [IMP]
- negative regulation of catalytic activity [ISS]
- negative regulation of macroautophagy [ISS]
- negative regulation of neuron apoptotic process [ISS]
- negative regulation of proteolysis [ISS]
- neuromuscular process controlling balance [ISS]
- neurotransmitter metabolic process [ISS]
- protein catabolic process [NAS]
- protein processing [ISS]
- receptor-mediated endocytosis [IMP]
- sphingomyelin metabolic process [IMP]
- vacuolar transport [IBA]
- vesicle transport along microtubule [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- Golgi membrane [IDA]
- Golgi stack [IDA]
- autophagic vacuole [ISS]
- caveola [IDA]
- cytoplasm [IDA]
- early endosome [IDA]
- endoplasmic reticulum [IDA]
- integral component of endoplasmic reticulum membrane [IDA]
- integral component of membrane [IDA]
- late endosome [IDA, ISS]
- lysosomal membrane [IDA]
- lysosome [IDA]
- membrane raft [IDA]
- mitochondrion [TAS]
- neuron projection [IDA]
- nucleus [IDA]
- plasma membrane [IDA]
- synaptic vesicle [IDA]
- trans-Golgi network [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.899624447 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 3.0 HEK 293T cells CompPASS score = 0.899624447, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HEK293T.
- This data may be re-scored from BioPlex 1.0 (PMID: 26186194) and BioPlex 2.0 (PMID: 28514442). Only scores from within the same cell line in BioPlex 3.0 (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID