Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Mapping of the interaction domain of the protein kinase CKII beta subunit with target proteins.

Ahn BH, Kim TH, Bae YS

Protein kinase CKII is composed of two catalytic (alpha or alpha') subunits and two regulatory (beta) subunits. The CKIIbeta subunit is thought to mediate the tetramer formation and interact with other target proteins. Previously we have shown that CKIIbeta interacts with ribosomal proteins L5 and L41, DNA topoisomerase IIbeta, and SAG/CKBBP1. In this study, the two-hybrid system was used to ... [more]

Mol. Cells Oct. 31, 2001; 12(2);158-63 [Pubmed: 11710515]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CSNK2B TOP2B
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3353242

Curated By

  • BioGRID