Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interactions of protein kinase CK2beta subunit within the holoenzyme and with other proteins.

Kusk M, Ahmed R, Thomsen B, Bendixen C, Issinger OG, Boldyreff B

Protein kinase CK2 is a ubiquitous, highly conserved protein kinase with a tetrameric alpha2beta2 structure. For the formation of this tetrameric complex a beta-alpha dimer seems to be a prerequisite. Using the two-hybrid system and a series of CK2beta deletion mutants, we mapped domains involved in alpha-beta and beta-beta interactions. We also detected an intramolecular beta interaction within the amino ... [more]

Mol. Cell. Biochem. Jan. 01, 1999; 191(1);51-8 [Pubmed: 10094392]

Throughput

  • Low Throughput

Curated By

  • BioGRID