BAIT

IRE1

ERN1, bifunctional endoribonuclease/protein kinase IRE1, L000000875, YHR079C
Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

ERV29

YGR284C
Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Heat shock response relieves ER stress.

Liu Y, Chang A

Accumulation of misfolded protein in the endoplasmic reticulum (ER) causes stress. The unfolded protein response (UPR), a transcriptional induction pathway, is activated to relieve ER stress. Although UPR is not essential for viability, UPR-deficient cells are more sensitive to ER stress; ire1Delta cells cannot grow when challenged with tunicamycin or by overexpression of misfolded CPY(*). In these cells, multiple functions ... [more]

EMBO J. Apr. 09, 2008; 27(7);1049-59 [Pubmed: 18323774]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: stress resistance (APO:0000080)

Additional Notes

  • Constitutively active Hsf1 rescues the growth defect of ire1 mutants on tunicamycin and CPY* overexpression. Suppression of the CPY* phenotype requires erv29, but the suppression by Hsf1 of the tunicamycin phenotype is not affected in ire1 erv29 mutants.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ERV29 IRE1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1476BioGRID
2124312
IRE1 ERV29
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1937BioGRID
2127410
ERV29 IRE1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2554BioGRID
2426798
IRE1 ERV29
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-5.3279BioGRID
209004
ERV29 IRE1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-5.3279BioGRID
208073

Curated By

  • BioGRID