BAIT
DUSP3
VHR
dual specificity phosphatase 3
GO Process (23)
GO Function (3)
GO Component (6)
Gene Ontology Biological Process
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- inactivation of MAPK activity [IMP]
- innate immune response [TAS]
- negative regulation of ERK1 and ERK2 cascade [IDA]
- negative regulation of JNK cascade [IDA, IMP]
- negative regulation of MAPK cascade [IMP]
- negative regulation of T cell activation [IDA]
- negative regulation of T cell receptor signaling pathway [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-tyrosine dephosphorylation [IDA]
- positive regulation of mitotic cell cycle [IMP]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GPX1
GPXD, GSHPX1
glutathione peroxidase 1
GO Process (21)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- UV protection [IMP]
- arachidonic acid metabolic process [TAS]
- cell redox homeostasis [IDA]
- cellular response to oxidative stress [NAS]
- glutathione metabolic process [IDA]
- heart contraction [IMP]
- hydrogen peroxide catabolic process [IDA]
- lipoxygenase pathway [TAS]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IMP]
- negative regulation of release of cytochrome c from mitochondria [IMP]
- nucleobase-containing small molecule metabolic process [TAS]
- positive regulation of fibril organization [ISS]
- purine nucleobase metabolic process [TAS]
- purine nucleotide catabolic process [TAS]
- regulation of gene expression, epigenetic [IDA]
- regulation of mammary gland epithelial cell proliferation [IMP]
- regulation of proteasomal protein catabolic process [IDA]
- response to hydrogen peroxide [IMP]
- response to selenium ion [IMP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.880258792 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.880258792, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID