BAIT

CG45263

43462, BcDNA:AT05815, BcDNA:GH22170, CG11718, CG11729, CG11730, CG11731, CG11733, CG31264, CG31369, CG31462, CG42285, CG42286, CG43462, CT32186, Dmel\CG45263, Dmel_CG11718, Dmel_CG31369, Dmel_CG31462, Dmel_CG42285, Dmel_CG42286, Dmel_CG43462, GH22170, GS3056, MRE1, anon-EST:Posey178, Dmel_CG45263

CG45263 gene product from transcript CG45263-RC

GO Process (0)

GO Function (0)

GO Component (0)

CRISPR Database FlyBase Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Drosophila melanogaster

PREY

VPS45

CG8228, DmVps45, Dmel\CG8228, MENE (3R)-C, MENE(3R)-C, Vps45p, dVps45, Dmel_CG8228

Vacuolar protein sorting 45

GO Process (9)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

endocytosis [IMP]

endosomal vesicle fusion [IMP]

intracellular protein transport [ISS]

lysosome organization [ISS]

neurotransmitter secretion [NAS]

plasma membrane to endosome transport [IMP]

protein targeting [ISS]

synaptic vesicle priming [NAS]

vesicle-mediated transport [ISS]

Gene Ontology Molecular Function

SNARE binding [IPI, ISS]
protein binding [IPI]

Gene Ontology Cellular Component

early endosome [IDA]

synaptic vesicle [NAS]

CRISPR Database FlyBase Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Drosophila melanogaster

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A protein interaction map of Drosophila melanogaster.

Giot L, Bader JS, Brouwer C, Chaudhuri A, Kuang B, Li Y, Hao YL, Ooi CE, Godwin B, Vitols E, Vijayadamodar G, Pochart P, Machineni H, Welsh M, Kong Y, Zerhusen B, Malcolm R, Varrone Z, Collis A, Minto M, Burgess S, McDaniel L, Stimpson E, Spriggs F, Williams J, Neurath K, Ioime N, Agee M, Voss E, Furtak K, Renzulli R, Aanensen N, Carrolla S, Bickelhaupt E, Lazovatsky Y, DaSilva A, Zhong J, Stanyon CA, Finley RL, White KP, Braverman M, Jarvie T, Gold S, Leach M, Knight J, Shimkets RA, McKenna MP, Chant J, Rothberg JM

Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence ... [more]

Science Dec. 05, 2003; 302(5651);1727-36 [Pubmed: 14605208]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
VPS45 CG45263	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Formstecher E (2005)	High	-	BioGRID	444038

Curated By

BioGRID

Interaction Summary