BAIT

VPS45

CG8228, DmVps45, Dmel\CG8228, MENE (3R)-C, MENE(3R)-C, Vps45p, dVps45, Dmel_CG8228
Vacuolar protein sorting 45
Drosophila melanogaster
PREY

CG45263

43462, BcDNA:AT05815, BcDNA:GH22170, CG11718, CG11729, CG11730, CG11731, CG11733, CG31264, CG31369, CG31462, CG42285, CG42286, CG43462, CT32186, Dmel\CG45263, Dmel_CG11718, Dmel_CG31369, Dmel_CG31462, Dmel_CG42285, Dmel_CG42286, Dmel_CG43462, GH22170, GS3056, MRE1, anon-EST:Posey178, Dmel_CG45263
CG45263 gene product from transcript CG45263-RC
GO Process (0)
GO Function (0)
GO Component (0)
Drosophila melanogaster

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Protein interaction mapping: a Drosophila case study.

Formstecher E, Aresta S, Collura V, Hamburger A, Meil A, Trehin A, Reverdy C, Betin V, Maire S, Brun C, Jacq B, Arpin M, Bellaiche Y, Bellusci S, Benaroch P, Bornens M, Chanet R, Chavrier P, Delattre O, Doye V, Fehon R, Faye G, Galli T, Girault JA, Goud B, de Gunzburg J, Johannes L, Junier MP, Mirouse V, Mukherjee A, Papadopoulo D, Perez F, Plessis A, Rosse C, Saule S, Stoppa-Lyonnet D, Vincent A, White M, Legrain P, Wojcik J, Camonis J, Daviet L

The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based technology to screen 102 bait proteins from Drosophila melanogaster, most of them orthologous to human cancer-related and/or signaling proteins, against high-complexity fly cDNA libraries. More than 2300 protein-protein interactions (PPI) were identified, of ... [more]

Genome Res. Mar. 01, 2005; 15(3);376-84 [Pubmed: 15710747]

Throughput

  • High Throughput

Additional Notes

  • Predicted Biological Score:D

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CG45263 VPS45
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID