Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Uncoupling proteins 2 and 3 interact with members of the 14.3.3 family.

Pierrat B, Ito M, Hinz W, Simonen M, Erdmann D, Chiesi M, Heim J

Uncoupling proteins (UCPs) are members of the superfamily of the mitochondrial anion carrier proteins (MATP). Localized in the inner membrane of the organelle, they are postulated to be regulators of mitochondrial uncoupling. UCP2 and 3 may play an important role in the regulation of thermogenesis and, thus, on the resting metabolic rate in humans. To identify interacting proteins that may ... [more]

Eur. J. Biochem. May. 01, 2000; 267(9);2680-7 [Pubmed: 10785390]

Throughput

  • Low Throughput

Curated By

  • BioGRID