BAIT

HLA-DQA1

CD, CELIAC1, DQ-A1, GSE, HLA-DQA, DAQB-109B10.1
major histocompatibility complex, class II, DQ alpha 1
Cell Cycle - Mitotic Spindle Assembly Project
Parkinson's Disease Project
Homo sapiens
PREY

HLA-DQB1

CELIAC1, HLA-DQB, IDDM1, DADB-249P12.2
major histocompatibility complex, class II, DQ beta 1
Parkinson's Disease Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

Huttlin EL, Bruckner RJ, Navarrete-Perea J, Cannon JR, Baltier K, Gebreab F, Gygi MP, Thornock A, Zarraga G, Tam S, Szpyt J, Gassaway BM, Panov A, Parzen H, Fu S, Golbazi A, Maenpaa E, Stricker K, Guha Thakurta S, Zhang T, Rad R, Pan J, Nusinow DP, Paulo JA, Schweppe DK, Vaites LP, Harper JW, Gygi SP

Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]

Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]

Quantitative Score

  • 0.999847476 [compPASS Score]

Throughput

  • High Throughput

Additional Notes

  • BioPlex HCT HCT116 cells CompPASS score = 0.999847476, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
  • Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HLA-DQB1 HLA-DQA1
Affinity Capture-Luminescence
Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

High-BioGRID
-
HLA-DQA1 HLA-DQB1
Affinity Capture-Luminescence
Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

High-BioGRID
-
HLA-DQA1 HLA-DQB1
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

High-BioGRID
2598501
HLA-DQB1 HLA-DQA1
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

High-BioGRID
2598538

Curated By

  • BioGRID