BAIT
METTL21B
FAM119B
methyltransferase like 21B
GO Process (0)
GO Function (0)
GO Component (1)
Gene Ontology Cellular Component
Homo sapiens
PREY
RPS6KA3
CLS, HU-3, ISPK-1, MAPKAPK1B, MRX19, RSK, RSK2, S6K-alpha3, p90-RSK2, pp90RSK2, RP11-393H10.3
ribosomal protein S6 kinase, 90kDa, polypeptide 3
GO Process (28)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- axon guidance [TAS]
- central nervous system development [TAS]
- innate immune response [TAS]
- negative regulation of apoptotic process [TAS]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of cell differentiation [TAS]
- positive regulation of cell growth [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of DNA-templated transcription in response to stress [TAS]
- regulation of translation in response to stress [TAS]
- response to lipopolysaccharide [ISS]
- signal transduction [TAS]
- skeletal system development [TAS]
- stress-activated MAPK cascade [TAS]
- synaptic transmission [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [ISS, TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [TAS]
- nucleoplasm [TAS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.990145287 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.990145287, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID