BAIT
AANAT
DSPS, SNAT
aralkylamine N-acetyltransferase
GO Process (7)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NACC2
BEND9, BTBD14, BTBD14A, BTBD31, NAC-2, RBB
NACC family member 2, BEN and BTB (POZ) domain containing
GO Process (10)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- cellular protein complex localization [IDA]
- histone deacetylation [IDA]
- negative regulation of G1/S transition of mitotic cell cycle by negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of transcription, DNA-templated [IDA, ISS]
- positive regulation of cell proliferation [ISS]
- positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage [IDA]
- posttranscriptional regulation of gene expression [IMP]
- protein homooligomerization [IDA, ISS]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IDA]
- histone deacetylase activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IDA]
- histone deacetylase activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [IDA]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.998137209 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.998137209, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID