BAIT

PAH1

SMP2, phosphatidate phosphatase PAH1, L000001934, YMR165C
Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; responsible for de novo lipid synthesis and formation of lipid droplets; phosphorylation by Pho80p-Pho85p decreases catalytic activity and alters Pah1p localization and abundance; phosphorylation by protein kinase A decreases catalytic efficiency; dephosphorylation by Nem1p-Spo7p anchors Pah1p to the membrane increasing substrate catalysis; homologous to mammalian lipins 1 and 2
Saccharomyces cerevisiae (S288c)
PREY

ARE1

SAT2, L000003221, L000002768, YCR048W
Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication
GO Process (1)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Production of ?-carotene in Saccharomyces cerevisiae through altering yeast lipid metabolism.

Zhao Y, Zhang Y, Nielsen J, Liu Z

Saccharomyces cerevisiae is a widely used cell factory for the production of fuels and chemicals. However, as a non-oleaginous yeast, S. cerevisiae has a limited production capacity for lipophilic compounds, such as ?-carotene. To increase its accumulation of ?-carotene, we engineered different lipid metabolic pathways in a ?-carotene producing strain and investigated the relationship between lipid components and the accumulation ... [more]

Biotechnol Bioeng Dec. 01, 2020; 118(5);2043-2052 [Pubmed: 33605428]

Throughput

  • Low Throughput

Ontology Terms

  • chemical compound excretion (APO:0000222)

Additional Notes

  • combined deletion of Pah1/Dpp1/Lpp1 AND overexpression are ARE1/ARE2 results in increased beta-carotene yields
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ARE1 PAH1
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
522279

Curated By

  • BioGRID