ATG13
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ATG5
Gene Ontology Biological Process
- C-terminal protein lipidation [IBA]
- autophagic vacuole assembly [IBA, ISS]
- autophagy [ISS]
- cellular response to nitrogen starvation [IBA]
- innate immune response [TAS]
- mitochondrion degradation [IBA]
- negative regulation of type I interferon production [TAS]
- nucleophagy [IBA]
- post-translational protein modification [ISS]
- regulation of cilium assembly [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
FIP200 regulates targeting of Atg16L1 to the isolation membrane.
Autophagosome formation is a dynamic process that is strictly controlled by autophagy-related (Atg) proteins. However, how these Atg proteins are recruited to the autophagosome formation site or autophagic membranes remains poorly understood. Here, we found that FIP200, which is involved in proximal events, directly interacts with Atg16L1, one of the downstream Atg factors, in an Atg14- and phosphatidylinositol 3-kinase-independent manner. ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID