PREY

HIF1A

HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
GO Process (34)
GO Function (13)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Alternative regulation of HIF-1? stability through Phosphorylation on Ser451.

Han HJ, Saeidi S, Kim SJ, Piao JY, Lim S, Guillen-Quispe YN, Choi BY, Surh YJ

The hypoxia-inducible factor (HIF-1?) functions as a master regulator of oxygen homeostasis. Oxygen-dependent hydroxylation of HIF-1? is tightly regulated by prolyl hydroxylase domain containing proteins (PHD1, PHD2, and PHD3). The prolyl hydroxylation facilitates the recruitment of the von Hippel-Lindau (VHL) protein, leading to ubiquitination and degradation of HIF-1? by the proteasomes. Besides prolyl hydroxylation, phosphorylation of HIF-1? is another central ... [more]

Biochem Biophys Res Commun Dec. 19, 2020; 545();150-156 [Pubmed: 33550096]

Throughput

  • Low Throughput

Additional Notes

  • Source of PIN1 not clear

Curated By

  • BioGRID